One of the enzyme that are involve in the ammonium assimilation is Asparagine synthetase (AS), some statements given below about AS such as,

a) This enzyme that catalyzes this reaction, is found in the cytosol of leaves and roots and in nitrogen-fixing nodules

b) High levels of light and carbohydrate inhibit the expression of genes coding for AS and the activity of the enzyme

c) Conditions of ample energy stimulate AS, thus they favor nitrogen assimilation into glutamine and glutamate

d) Energy-limited conditions inhibit GS and GOGAT, stimulate AS, and thus favor nitrogen assimilation into asparagine, a compound that is rich in nitrogen and sufficiently stable for long-distance transport or long term storage

Which of the following statements about AS is correct?

TLS Online TPP Program

#Question id: 23147

#Applied Microbiology

In fluorescence microscopy why the fluorescence occurs at longer wavelength when there shorter   wavelength of light is applied?

TLS Online TPP Program

#Question id: 23148

#Applied Microbiology

There are two proteins A and B to be visualized under fluorescence microscopy. Both of them are excited with same wavelength of light but A emits longer wavelength. Hence we can conclude that 

1. A is denser molecule than B

2. B is denser molecule than A

3. A is lighter molecule than B

4. B is lighter molecule than A

Which of the following option is incorrect?

TLS Online TPP Program

#Question id: 23156

#Applied Microbiology

Most widely used method of detecting specific proteins, uses an antibody to which a fluorescent dye has been covalently attached

TLS Online TPP Program

#Question id: 23157

#Applied Microbiology

Immunofluorescence microscopy, the most widely used method of detecting 

TLS Online TPP Program

#Question id: 23158

#Applied Microbiology

You have purified protein X and injected into an animal so that over a period of weeks, the animal will mount an immune response and make antibodies to protein X. By which technique these generated antibodies can be extracted from blood serum.

TLS Online TPP Program

#Question id: 23168

#Applied Microbiology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?