#Question id: 40429
#Applied Microbiology
Read the following statements regarding chromatography techniques:
Statement I: Ion exchange chromatography separates molecules based on differences in their net surface charge.
Statement II: Gel filtration chromatography (Size Exclusion) separates molecules based on their specific binding to a ligand.
Choose the correct answer from the options given below:
#Question id: 40430
#Applied Microbiology
Assertion (A): Total cellular RNA passed through an oligo(dT) column results in the specific elution of mRNA.
Reason (R): Prokaryotic rRNA and tRNA constitute the majority of total RNA and they lack a 3' Poly-A tail.
Choose the correct option:
#Question id: 40431
#Applied Microbiology
Match the Chromatography Type (List I) with its Principle of Separation (List II).
| List I (Chromatography) | List II (Basis of Separation) |
| (a) Ion Exchange | (i) Hydrophobicity |
| (b) Gel Filtration | (ii) Specific Ligand Interaction |
| (c) Affinity | (iii) Net Charge |
| (d) Reverse Phase | (iv) Molecular Size / Stokes Radius |
#Question id: 40432
#Botany
| LIST-I (Enzyme) | LIST-II (Activator/Co-factor) |
| A. Nitrogenase | I. Magnesium (Mg²⁺) |
| B. RuBisCO | II. Molybdenum (Mo) |
| C. Alcohol Dehydrogenase | III. Iron (Fe) |
| D. Ferredoxin | IV. Zinc (Zn²⁺) |
#Question id: 40433
#Botany
#Question id: 40434
#Botany
