TLS Online TPP Program
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TLS Online TPP Program
#Question id: 12911
#Applied Microbiology
Match the following:
A. DNA polymerases i. Alkaline phosphatase a.RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase ii. T4 DNA ligase b. Thermostable DNA polymerase used in PCR
C. Ligases iii. AMV-RT c. Removes 5′-phosphates from DNA and RNA
D. Phosphatases iv. Taq DNA pol d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond
TLS Online TPP Program
#Question id: 12912
#Applied Microbiology
Name the enzyme which separates the double-helical DNA into single strands.
TLS Online TPP Program
#Question id: 12925
#Applied Microbiology
Another name of TaqMan assay is ____
TLS Online TPP Program
#Question id: 12932
#Applied Microbiology
Why mRNA cannot be used directly in cloning?
TLS Online TPP Program
#Question id: 12933
#Applied Microbiology
Choose the correct order for the cloning process.
1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.
2. mRNA cannot be used directly in cloning.
3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.
4. The cDNA may be inserted into vectors and then cloned.
TLS Online TPP Program
#Question id: 12934
#Applied Microbiology
Reverse transcriptase enzyme is isolated from____