TLS Online TPP Program

#Question id: 12946


Choose the incorrect statement for the Multiple Ligation dependent probe amplification.

#Zoology
  1. In this method two hybridization probes are used.
  2. One containing a forward PCR primer sequence labelled with a fluorescent dye.
  3. Other with a stuffer sequence and reverse primer sequence.
  4. If ligation takes place then PCR amplification cannot proceed.
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TLS Online TPP Program

#Question id: 37995

#Plant Biotechnology

List I (Method)List II (Detail)
A. Colchicine-lanolin paste1. Anthers plated on supplemented medium for 1 week
B. Repeated axillary bud treatment2. Main axis decapitated to stimulate axillary buds
C. Anther Culture3. Cotton plugs applied for 14 days (e.g., Potato)

TLS Online TPP Program

#Question id: 37996

#Plant Biotechnology

Which of the following conditions is crucial for "Post-treatment care" after applying colchicine to plants? 

TLS Online TPP Program

#Question id: 37997

#Plant Biotechnology

For the "Plantlet treatment" method, what is the standard concentration and duration of colchicine exposure described? 

TLS Online TPP Program

#Question id: 37998

#Plant Biotechnology

Statement I: Unpollinated ovaries are generally preferred over pollinated ones for the production of haploids via gynogenesis.
Statement II: In maize, diploidization issues during anther culture are often addressed by treating the crowns of 3–4 tiller stage plants with DMSO.

Choose the correct answer: 

TLS Online TPP Program

#Question id: 37999

#Plant Biotechnology

Statement I: For gynogenesis, complete ovule maturation is strictly necessary for successful induction.
Statement II: Unpollinated ovaries are generally preferred over pollinated ones for haploid production.

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TLS Online TPP Program

#Question id: 38000

#Plant Biotechnology

List I (Factor)List II (Effect/Detail)
A. Seasonal Effects1. Ovule callus induction in Gerbera is higher in autumn
B. Stage of Collection2. Flower buds excised 24–48 hours before anthesis
C. Physical Factors3. Cold treatment increases haploid callus frequency in Salvia sclarea