TLS Online TPP Program

#Question id: 12946


Choose the incorrect statement for the Multiple Ligation dependent probe amplification.

#Applied Microbiology
  1. In this method two hybridization probes are used.
  2. One containing a forward PCR primer sequence labelled with a fluorescent dye.
  3. Other with a stuffer sequence and reverse primer sequence.
  4. If ligation takes place then PCR amplification cannot proceed.
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TLS Online TPP Program

#Question id: 38806

#Plant Biotechnology

Q. Thermotherapy is noted to be effective against which specific types of pathogens? 

TLS Online TPP Program

#Question id: 38807

#Plant Biotechnology

Q. Why might a plant that survives thermotherapy be at a disadvantage later on?

TLS Online TPP Program

#Question id: 38808

#Plant Biotechnology

Q. Assertion (A):Thermotherapy combined with meristem (shoot-tip) culture is more effective than either method alone for eliminating certain plant viruses.

Reason (R): Some viruses such as Potato virus S (PVS) and Potato virus X (PVX) can only be eliminated when heat treatment is followed by shoot-tip culture, and larger shoot tips taken from heat-treated plants show better survival.

Choose the correct option:

TLS Online TPP Program

#Question id: 38809

#Plant Biotechnology

Q. Assertion (A): Prolonged cold treatment followed by shoot-tip culture can successfully eliminate plant viruses.

Reason (R):Exposure of chrysanthemum plants to 5°C for several months increased the proportion of virus-free plants for both chrysanthemum stunt virus and chrysanthemum chlorotic mottle virus.

TLS Online TPP Program

#Question id: 38810

#Plant Biotechnology

Q. Assertion (A):Increasing the duration of cryotherapy improves virus elimination efficiency.

Reason (R):Extending cold treatment at 5°C from 4 months to 9.5 months increased virus-free frequencies for both CSV and CCMV.

TLS Online TPP Program

#Question id: 38811

#Plant Biotechnology

Q. Assertion (A):Cryotherapy is generally more damaging to plants than thermotherapy.

Reason (R):Cold treatment causes greater physiological stress compared to heat treatment.