Choose the correct order for the steps involve in Insitu hybridization.

1. Cell permeaabilisation

2. Detection of hybridised probe radioactive.

3. Wash excess probe from section

4. Cell fixation

TLS Online TPP Program

#Question id: 40231

#Botany

Match List-I with List-II

LIST-I (Plant Name)	LIST-II (Family)
A. Arachis hypogaea (Groundnut)	I. Pedaliaceae
B. Sesamum indicum (Sesame)	II. Malvaceae
C. Linum usitatissimum (Flax)	III. Fabaceae
D. Gossypium hirsutum (Cotton)	IV. Linaceae

Choose the correct answer from the options given below:

TLS Online TPP Program

#Question id: 40232

#Applied Microbiology

In SDS-PAGE, which of the following reagents is specifically responsible for unfolding the tertiary structure of proteins by coating them with a uniform negative charge?

TLS Online TPP Program

#Question id: 40233

#Applied Microbiology

Consider the following statements regarding vertical gel electrophoresis (SDS-PAGE):

Statement I: The separation of proteins in SDS-PAGE is primarily based on their intrinsic isoelectric point (pI).

Statement II: Dithiothreitol (DTT) can be used as an alternative to beta-mercaptoethanol to achieve protein reduction.

TLS Online TPP Program

#Question id: 40234

#Applied Microbiology

Assertion (A): In the absence of beta-mercaptoethanol, a multimeric protein linked by disulfide bridges will migrate as a single large band.

Reason (R): beta-mercaptoethanol acts by oxidizing the thiol groups of cysteine residues to form stable covalent bonds.

TLS Online TPP Program

#Question id: 40235

#Applied Microbiology

Match the reagent with its specific role in vertical gel electrophoresis:

List-I (Reagent)	List-II (Function)
(A). Glycerol	(I). Catalyst for polymerization
(B). Ammonium Persulfate (APS)	(II). Increases sample density for loading
(C). Glycine	(III). Provides buffering ions in the running buffer
(D). Polyacrylamide	(IV). Acts as the supporting matrix (sieve)

TLS Online TPP Program

#Question id: 40236

#Botany

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).

Assertion (A): The roots of Rauvolfia serpentina are used effectively in the treatment of hypertension (high blood pressure).

Reason (R): The plant contains the indole alkaloid Reserpine, which acts on the nervous system to lower blood pressure

In the light of the above statements, choose the most appropriate answer from the options given below: