TLS Online TPP Program

#Question id: 3667


In an ______________ plasmid, replication occurs in two stages. In the first stage, the double stranded circular plasmid DNA replicates to form another double-stranded circular DNA and a single-stranded circular DNA, In the second stage, the complementary strand is synthesized on the single-stranded DNA to make another double-stranded DNA.

#Life Sciences
  1. all

  2. some

  3. rolling-circle

  4. most

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TLS Online TPP Program

#Question id: 40458

#Applied Microbiology

Consider the following statements regarding the principles of electrophoresis:

    Statement I: Electrophoresis separates molecules primarily based on their charge-to-mass ratio.

    Statement II: Neutral molecules with zero net charge remain stationary or move randomly in an electric field.

    Which of the above statements is/are correct?

    TLS Online TPP Program

    #Question id: 40459

    #Applied Microbiology

      Assertion (A): Standard Gel Electrophoresis is rarely used for the separation of simple lipids.

        Reason (R): Lipids are biomolecules that are primarily hydrophobic and lack the net charge required for migration in an aqueous electric field.

        Choose the correct option:

        TLS Online TPP Program

        #Question id: 40460

        #Applied Microbiology

        Match the biomolecule in Column I with the standard separation/visualization medium used in Column II.

        Column I (Biomolecule)Column II (Method/Medium)
        A. DNA fragments1. Polyacrylamide Gel (PAGE)
        B. Proteins2. Agarose Gel
        C. Plant Pigments/Lipids3. Paper Chromatography / TLC

        TLS Online TPP Program

        #Question id: 40461

        #Applied Microbiology

        In the E. coli chemotaxis pathway, which of the following events directly leads to "tumbling" behavior?

        TLS Online TPP Program

        #Question id: 40462

        #Applied Microbiology

        Consider the following statements regarding the Adaptation phase of chemotaxis:

        Statement I: Adaptation allows bacteria to reset their sensitivity to attractant concentrations, enabling them to detect changes over a wide range.

        Statement II: The protein CheB acts as a methylesterase that removes methyl groups from the MCP receptors, while CheR acts as a methyltransferase.

        Which of the above statements is/are correct?

        TLS Online TPP Program

        #Question id: 40463

        #Applied Microbiology

      • Assertion (A): In the presence of a high concentration of an attractant, the frequency of bacterial tumbling decreases (longer runs).

      • Reason (R): Attractant binding causes a conformational change in the receptor that inhibits CheA kinase activity, leading to reduced levels of CheY-P.