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TLS Online TPP Program

#Question id: 541

The overall △G for glycolysis is -72 kJ/mol in erythrocytes. Which statement below is true?

A) The value of △G0ʹ is also -72 kJ/mol since the cytosol pH is close to 7.

B) The free energy of glycolysis is found as the sum of the standard free energy changes for

the individual pathway reactions.

C) The negative sign of △G shows that this pathway will proceed toward product (pyruvate) under normal cellular conditions.

#Biochemistry

  1. A Only   

  2. A&B Only  

  3. A&C only 

  4. C Only

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TLS Online TPP Program

#Question id: 40201

#Environmental Science

Match List I (Atmospheric Layers) with List II (Characteristics):

List I (Layer)List II (Characteristic)
A. TroposphereI. Reflects radio waves
B. StratosphereII. Lapse rate is positive (Temperature decreases with height)
C. ThermosphereIII. Contains the Ozone layer
D. IonosphereIV. Temperature increases significantly with height due to solar radiation
TLS Online TPP Program

#Question id: 40202

#Applied Microbiology

Assertion (A): Primers with high GC content generally have a higher melting temperature (Tm) than AT-rich primers of the same length.

Reason (R): Guanine and Cytosine are bonded by three hydrogen bonds, which require more energy to break than the two hydrogen bonds between Adenine and Thymine
TLS Online TPP Program

#Question id: 40203

#Applied Microbiology

Which cofactor is essential for the activity of Taq DNA polymerase during the PCR extension step?
TLS Online TPP Program

#Question id: 40204

#Applied Microbiology

Match the PCR component in Column A with its specific function in Column B

Column A (Component)Column B (Function)
1. dNTPsA. Provides the starting point for DNA synthesis
2. PrimersB. Building blocks for the new DNA strand
3. BufferC. Maintains pH and salt conditions
4. Template DNAD. Contains the target region to be amplified
TLS Online TPP Program

#Question id: 40205

#Applied Microbiology

Read the following statements regarding PCR and choose the correct option.

Statement I: DNA synthesis during PCR always proceeds in the 3' ---------5' direction.

Statement II: If the annealing temperature is set too low, it often leads to non-specific binding of primers and amplification of incorrect products. 
TLS Online TPP Program

#Question id: 40206

#Applied Microbiology

Assertion (A): Taq polymerase is preferred over normal DNA polymerases (like E. coli DNA Pol I) for PCR.

Reason (R): Taq polymerase has a proofreading activity (3'-------5'exonuclease) that ensures zero errors during amplification.