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TLS Online TPP Program

#Question id: 20289

Which of the following is INCORRECT about insulinoma?

#Biochemistry
  1. It is much rarer than diabetes excessive insulin production occasionally occurs from an adenoma of an islet of Langerhans.
  2. About 10 to 15 percent of these adenomas are malignant, and occasionally metastases from the islets of Langerhans spread throughout the body.
  3. Indeed, more than 1000 grams of glucose have had to be administered every 24 hours to prevent hypoglycemia in some of these patients.
  4. The pancreatic beta cells become “exhausted” or damaged and are unable to produce enough insulin to prevent more severe hyperglycemia.
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TLS Online TPP Program

#Question id: 40210

#Applied Microbiology

Match the PCR step in Column I with the correct temperature range or characteristic in Column II.

 Column I (PCR Step) Column II (Condition / Event)
 A. Denaturation 1. ~72 °C; Optimum for Taq polymerase activity
 B. Annealing 2. ~94 °C; Hydrogen bonds break to separate DNA strands 
 C. Extension 3. ~4 °C; Short-term storage after cycling
 D. Final Hold 4. ~50–65 °C; Primers bind to the template
TLS Online TPP Program

#Question id: 40211

#Environmental Science

 Match the scientists in List I with their proposed theories or experiments in List II.

List I (Scientists)List II (Theories/Experiments)
A. A.I. Oparin & J.B.S. HaldaneI. Swan-necked flask experiment (Biogenesis)
B. S.L. Miller & H.C. UreyII. Coacervate droplets (Chemical Evolution)
C. Louis PasteurIII. Microspheres (Thermal synthesis of proteins)
D. Sidney FoxIV. Simulation of primitive earth conditions
TLS Online TPP Program

#Question id: 40212

#Applied Microbiology

Read the following statements regarding the steps of a standard PCR cycle and choose the correct option.

Statement I: The denaturation step involves the breaking of phosphodiester bonds to separate the double-stranded DNA into single strands.

Statement II: The annealing temperature is usually set 3 -5 0   C lower than the melting temperature (Tm) of the primers to ensure specific binding.
TLS Online TPP Program

#Question id: 40213

#Applied Microbiology

In the following question, a statement of Assertion (A) is followed by a statement of Reason (R).

Assertion (A): The annealing step must be performed at a significantly lower temperature than the denaturation step.

Reason (R): High temperatures destabilize hydrogen bonds, while lower temperatures favor the formation of hydrogen bonds required for primers to hybridize with the template.
TLS Online TPP Program

#Question id: 40214

#Applied Microbiology

Which of the following represents the correct sequence of temperature changes during one single cycle of PCR amplification? 
TLS Online TPP Program

#Question id: 40215

#Environmental Science

Match the geological eons/events in List I with the biological milestones in List II.

List I (Time Period/Event)List II (Biological Milestone)
A. 4.0 - 3.8 Billion years agoI. Appearance of first Eukaryotes
B. 3.5 Billion years agoII. Oxygen Revolution (Great Oxidation Event)
C. 2.7 - 2.5 Billion years agoIII. Appearance of first cellular life (Prokaryotes)
D. 2.0 - 1.5 Billion years agoIV. Formation of life precursors (RNA/Protocells)