TLS Online TPP Program
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TLS Online TPP Program
#Question id: 38765
#Plant Biotechnology
Q. Why is the "washing process" considered a critical step for actual fusion to occur?
TLS Online TPP Program
#Question id: 38766
#Plant Biotechnology
Q. Statement 1 (Assertion): Protoplasts located in close proximity are more likely to undergo fusion during the washing phase of a PEG treatment.
Statement 2 (Reason): The removal of PEG molecules disrupts the organized structure of the plasma lemma, creating localized instabilities that facilitate membrane merging
Which of the following is correct?
TLS Online TPP Program
#Question id: 38767
#Plant Biotechnology
Q. Statement I: Polyethylene glycol (PEG) facilitates protoplast fusion by disturbing the plasma membrane organization.
Statement II: PEG can bind Ca²⁺ ions, which act as bridges between negatively charged PEG and plasma membrane components.
TLS Online TPP Program
#Question id: 38768
#Plant Biotechnology
Q. Statement I: PEG is negatively charged and can interact with cations such as Ca²⁺.
Statement II: Ca²⁺ ions can bind to negatively charged molecules on the plasma membrane, promoting membrane proximity.
TLS Online TPP Program
#Question id: 38769
#Plant Biotechnology
Q. Statement I: During the washing step, PEG may remove plasma membrane components bound to it.
Statement II: Removal of these components can destabilize membrane integrity and promote fusion of adjacent protoplasts.
TLS Online TPP Program
#Question id: 38770
#Plant Biotechnology
Q. Statement I: PEG-induced fusion is a consequence of controlled membrane damage and reorganization.
Statement II: Plasma membrane destabilization increases the likelihood of fusion between closely placed protoplasts.