Q. Which of the following is an advantage of the mechanical method over enzymatic methods?

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#Question id: 38713

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Q.  Assertion: The enzymatic method is superior to the mechanical method for large-scale experiments. 

Reason: Enzymatic isolation provides high yield and good viability of protoplasts.

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#Question id: 38714

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Q. Assertion: Barley aleurone cells require treatment with glusulase to release protoplasts. 

Reason: Treatment of these cells with cellulase results in sphaeroplasts, which still possess a residual cell wall.

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#Question id: 38715

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Q. The enzymatic method of protoplast isolation, introduced by Cocking in 1960, primarily uses which enzyme for cell wall degradation? 

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#Question id: 38716

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Q. Which physiological condition is most ideal for isolating protoplasts from callus tissue? 

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#Question id: 38717

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Q. In the sequential (two-step) enzymatic isolation method, what is the correct order of enzyme treatment?

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#Question id: 38718

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Q. Failure of sustained cell division in isolated protoplasts is MOST likely due to