Q. With reference to feeder layer technique in protoplast culture:

1. Feeder cells provide diffusible growth factors.

2. It compensates for the absence of cell-to-cell signaling.

3. Feeder cells directly fuse with protoplasts.

4. It increases plating efficiency of isolated protoplasts.

Which statements are correct?

TLS Online TPP Program

#Question id: 37831

#Plant Biotechnology

Assertion (A): In plant tissue culture, only tissues with an intact membrane system and viable nucleus can regenerate into a whole plant.

Reason (R): The presence of chloroplasts is essential for dedifferentiation and regeneration.

TLS Online TPP Program

#Question id: 37832

#Plant Biotechnology

What is cellular totipotency in plants?

TLS Online TPP Program

#Question id: 37833

#Plant Biotechnology

Which of the following are examples of totipotent cells?

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#Question id: 37834

#Plant Biotechnology

Two key phenomena in cellular totipotency that allow plant regeneration are:

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#Question id: 37835

#Plant Biotechnology

Which statement is TRUE about plant cellular totipotency?

TLS Online TPP Program

#Question id: 37836

#Plant Biotechnology

Column A	Column B
1. Dry Heat	A. 121 °C at 15 PSI for 15–20 min
2. Autoclaving	B. 0.45 μm pore size membrane
3. Filter Sterilization	C. 160–180°C for 3 hours
4. Laminar Air Flow	D. HEPA filter (removes particles > 0.3 μm)