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TLS Online TPP Program

#Question id: 4769

In some sheep, the presence of horns is produced by an autosomal allele that is dominant in males and recessive in females. A horned female is crossed with a hornless male. One of the resulting F1 females is crossed with a hornless male. What proportion of the male and female progeny from this cross will have horns?

#Applied Microbiology

  1. Half of the male progeny will be horned but none of the female progeny will be horned

  2. Half of the male progeny will be horned and half of the female progeny will be horned

  3. none of the male progeny will be horned but half of the female progeny will be horned

  4. All of the male progeny will be horned but none of the female progeny will be horned

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TLS Online TPP Program

#Question id: 40199

#Applied Microbiology

Match the parameter in Column A with its formula or definition in Column B

Column A (Parameter)Column B (Definition/Formula)
1. Dilution Rate (D)A. ln (2) / mu
2. Doubling Time (td)B. Flow rate (F) / Volume (V)
3. Yield Coefficient (Y)C. Change in cell mass / Change in substrate consumed
4. Specific Growth Rate (mu)D. 1/x . dx/dt
TLS Online TPP Program

#Question id: 40200

#Applied Microbiology

Calculate the melting temperature (Tm) for the following primer sequence using the Wallace Rule

Sequence: 5'-GGCCAAAATTTTGGCC-3'
TLS Online TPP Program

#Question id: 40201

#Environmental Science

Match List I (Atmospheric Layers) with List II (Characteristics):

List I (Layer)List II (Characteristic)
A. TroposphereI. Reflects radio waves
B. StratosphereII. Lapse rate is positive (Temperature decreases with height)
C. ThermosphereIII. Contains the Ozone layer
D. IonosphereIV. Temperature increases significantly with height due to solar radiation
TLS Online TPP Program

#Question id: 40202

#Applied Microbiology

Assertion (A): Primers with high GC content generally have a higher melting temperature (Tm) than AT-rich primers of the same length.

Reason (R): Guanine and Cytosine are bonded by three hydrogen bonds, which require more energy to break than the two hydrogen bonds between Adenine and Thymine
TLS Online TPP Program

#Question id: 40203

#Applied Microbiology

Which cofactor is essential for the activity of Taq DNA polymerase during the PCR extension step?
TLS Online TPP Program

#Question id: 40204

#Applied Microbiology

Match the PCR component in Column A with its specific function in Column B

Column A (Component)Column B (Function)
1. dNTPsA. Provides the starting point for DNA synthesis
2. PrimersB. Building blocks for the new DNA strand
3. BufferC. Maintains pH and salt conditions
4. Template DNAD. Contains the target region to be amplified