#Question id: 40230
#Applied Microbiology
Match the specialized structures/enzymes with the corresponding nitrogen fixer:
| List-I (Structure/Enzyme) | List-II (Microorganism) |
| (A). Heterocyst | (I). Rhizobium |
| (B). Leghaemoglobin | (II). Anabaena |
| (C). Respiratory Protection | (III). Clostridium |
| (D). Anaerobic N-fixation | (IV). Azotobacter |
#Question id: 40231
#Botany
| LIST-I (Plant Name) | LIST-II (Family) |
| A. Arachis hypogaea (Groundnut) | I. Pedaliaceae |
| B. Sesamum indicum (Sesame) | II. Malvaceae |
| C. Linum usitatissimum (Flax) | III. Fabaceae |
| D. Gossypium hirsutum (Cotton) | IV. Linaceae |
#Question id: 40232
#Applied Microbiology
In SDS-PAGE, which of the following reagents is specifically responsible for unfolding the tertiary structure of proteins by coating them with a uniform negative charge?
#Question id: 40233
#Applied Microbiology
Consider the following statements regarding vertical gel electrophoresis (SDS-PAGE):
Statement I: The separation of proteins in SDS-PAGE is primarily based on their intrinsic isoelectric point (pI).
Statement II: Dithiothreitol (DTT) can be used as an alternative to beta-mercaptoethanol to achieve protein reduction.
#Question id: 40234
#Applied Microbiology
Assertion (A): In the absence of beta-mercaptoethanol, a multimeric protein linked by disulfide bridges will migrate as a single large band.
Reason (R): beta-mercaptoethanol acts by oxidizing the thiol groups of cysteine residues to form stable covalent bonds.
#Question id: 40235
#Applied Microbiology
Match the reagent with its specific role in vertical gel electrophoresis:
| List-I (Reagent) | List-II (Function) |
| (A). Glycerol | (I). Catalyst for polymerization |
| (B). Ammonium Persulfate (APS) | (II). Increases sample density for loading |
| (C). Glycine | (III). Provides buffering ions in the running buffer |
| (D). Polyacrylamide | (IV). Acts as the supporting matrix (sieve) |