Q. Regarding cybridization for male sterile line development:

Statement I: Cybrids allow rapid transfer of mitochondria even across sexually incompatible species.

Statement II: Male sterile lines developed via protoplast fusion can be used for hybrid seed production using CMS restorers.

Statement III: Nuclear genomes are exchanged between donor and recipient protoplasts in cybridization.

Which statements are correct?

TLS Online TPP Program

#Question id: 39284

#Plant Biotechnology

Q. Statement I: Fusing tomato protoplasts with irradiated Solanum acaule protoplasts produced plants that were morphologically identical to Solanum acaule. 

Statement II: The resulting tomato regenerants exhibited Cytoplasmic Male Sterility (CMS), which was inherited maternally.

TLS Online TPP Program

#Question id: 39285

#Plant Biotechnology

Q. Statement I: Protoplast fusion can be used to develop male sterile lines even between sexually incompatible species. 

Statement II: CMS lines developed through this method cannot be used with traditional CMS restorers.

TLS Online TPP Program

#Question id: 39286

#Plant Biotechnology

Q. Assertion: Cybrids can be produced by the irradiation of protoplasts from one of the fusion parents. 

Reason: Irradiation effectively inactivates the nucleus of the donor protoplast, allowing only its cytoplasm to contribute to the fusion product.

TLS Online TPP Program

#Question id: 39287

#Plant Biotechnology

Q. Assertion: Cybridization is a valuable tool for transferring traits like cytoplasmic male sterility (CMS) and herbicide resistance in crops like rice and tobacco. 

Reason: Cybrids contain the nuclear genome of only one species, which ensures the plant retains its primary species characteristics while gaining specific cytoplasmic traits.

TLS Online TPP Program

#Question id: 39288

#Plant Biotechnology

Q. Assertion: Cybrids allow for the combination of mitochondria from one species with chloroplasts from a different species.

 Reason: This combination can be used to overcome deleterious effects, such as chlorophyll deficiency, found in some alloplasmic lines.

TLS Online TPP Program

#Question id: 39289

#Plant Biotechnology

Q. Assertion:Brassica napus lines carrying Ogura (radish) cytoplasm are highly efficient for commercial growth in cold climates without any modifications. 

Reason: The presence of radish chloroplasts in these lines causes chlorophyll deficiency under low temperatures.