Q. Statement I: Conventional backcrossing is a more time-efficient method for cytoplasm transfer compared to somatic hybridization. 

Statement II: Protoplast fusion can achieve cytoplasm transfer in approximately one year.

#Question id: 40202

#Applied Microbiology

Assertion (A): Primers with high GC content generally have a higher melting temperature (Tm) than AT-rich primers of the same length.

Reason (R): Guanine and Cytosine are bonded by three hydrogen bonds, which require more energy to break than the two hydrogen bonds between Adenine and Thymine

#Question id: 40203

#Applied Microbiology

Which cofactor is essential for the activity of Taq DNA polymerase during the PCR extension step?

#Question id: 40204

#Applied Microbiology

Match the PCR component in Column A with its specific function in Column B

#Question id: 40205

#Applied Microbiology

Read the following statements regarding PCR and choose the correct option.

Statement I: DNA synthesis during PCR always proceeds in the 3' ---------5' direction.

Statement II: If the annealing temperature is set too low, it often leads to non-specific binding of primers and amplification of incorrect products. 

#Question id: 40206

#Applied Microbiology

Assertion (A): Taq polymerase is preferred over normal DNA polymerases (like E. coli DNA Pol I) for PCR.

Reason (R): Taq polymerase has a proofreading activity (3'-------5'exonuclease) that ensures zero errors during amplification.

#Question id: 40207

#Environmental Science