TLS Online TPP Program

#Question id: 39300


Q. Which parental combinations were used to produce CMS (cytoplasmic male sterility)?

#Plant Biotechnology
  1. N. tabacum + N. sylvestris
  2. B. campestris + B. napus
  3. Oryza spp.
  4. All of the above
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TLS Online TPP Program

#Question id: 40197

#Applied Microbiology

Assertion (A): A turbidostat is most effective when operating at high dilution rates near the maximum specific growth rate (mumax}).

Reason (R): At low dilution rates, the chemostat is more stable because the concentration of the limiting nutrient is easier to control than turbidity

TLS Online TPP Program

#Question id: 40198

#Applied Microbiology

Read the following statements regarding Fed-Batch culture and choose the correct option.

Statement I: Fed-batch culture is ideal for producing secondary metabolites (like antibiotics) because it allows the culture to be maintained in the stationary phase for a prolonged period.

Statement II: In fed-batch culture, the volume of the culture broth remains constant throughout the fermentation process. 

TLS Online TPP Program

#Question id: 40199

#Applied Microbiology

Match the parameter in Column A with its formula or definition in Column B

Column A (Parameter)Column B (Definition/Formula)
1. Dilution Rate (D)A. ln (2) / mu
2. Doubling Time (td)B. Flow rate (F) / Volume (V)
3. Yield Coefficient (Y)C. Change in cell mass / Change in substrate consumed
4. Specific Growth Rate (mu)D. 1/x . dx/dt

TLS Online TPP Program

#Question id: 40200

#Applied Microbiology

Calculate the melting temperature (Tm) for the following primer sequence using the Wallace Rule

Sequence: 5'-GGCCAAAATTTTGGCC-3'

TLS Online TPP Program

#Question id: 40201

#Environmental Science

Match List I (Atmospheric Layers) with List II (Characteristics):

List I (Layer)List II (Characteristic)
A. TroposphereI. Reflects radio waves
B. StratosphereII. Lapse rate is positive (Temperature decreases with height)
C. ThermosphereIII. Contains the Ozone layer
D. IonosphereIV. Temperature increases significantly with height due to solar radiation

TLS Online TPP Program

#Question id: 40202

#Applied Microbiology

Assertion (A): Primers with high GC content generally have a higher melting temperature (Tm) than AT-rich primers of the same length.

Reason (R): Guanine and Cytosine are bonded by three hydrogen bonds, which require more energy to break than the two hydrogen bonds between Adenine and Thymine