TLS Online TPP Program

#Question id: 71


Which of the following is not the priority rule for R, S Configuration?

#Biochemistry
  1. If the four atoms attached to the chiral center are all different, priority depends on atomic number, with the atom of lower atomic number getting lower priority.

  2. If the two atoms attached to chiral centers are same, the atoms attached to each of these first atoms are compared

  3. When there is a double bond or triple bond, both atoms are considered to be duplicated or triplicated

  4. If the four atoms attached to the chiral center are all different, priority depends on atomic number, with the atom of higher atomic number getting lower priority.

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TLS Online TPP Program

#Question id: 38771

#Plant Biotechnology

Q. Statement I: PEG can interact with both negatively and positively charged components of the plasma membrane.
Statement II: Such interactions reduce electrostatic repulsion between protoplasts.

TLS Online TPP Program

#Question id: 38772

#Plant Biotechnology

Q. Statement I: Protoplasts are isolated by enzymatic digestion of the cell wall using a combination of cellulase and pectinase.
Statement II: Cellulose microfibrils and pectic substances are major structural components of plant cell walls.

TLS Online TPP Program

#Question id: 38773

#Plant Biotechnology

Q.Statement I: The pH of the enzyme solution for protoplast isolation is maintained between 4.7 and 8.0.
Statement II: Enzymes involved in cell wall degradation show activity only at neutral pH.

TLS Online TPP Program

#Question id: 38774

#Plant Biotechnology

Q. Statement I: Protoplast isolation is usually carried out at a temperature range of 25–30°C.

Statement II: This temperature range supports optimal enzyme activity and maintains protoplast viability.


TLS Online TPP Program

#Question id: 38775

#Plant Biotechnology

Q. Statement I: High osmotic concentration of the enzyme mixture is maintained using sorbitol or mannitol.
Statement II: Elevated osmoticum prevents protoplast bursting by balancing internal turgor pressure.

TLS Online TPP Program

#Question id: 38776

#Plant Biotechnology

Q. Statement I: Calcium chloride is added to the osmoticum during protoplast isolation.
Statement II: Calcium ions improve plasma membrane stability of protoplasts.