#Question id: 40219
#Environmental Science
Match the evolutionary stages in List I with their defining characteristics in List II
| List I (Evolutionary Stage) | List II (Characteristics) |
| A. Chemoheterotrophs | I. First oxygen-producing photosynthesizers (Cyanobacteria) |
| B. Chemoautotrophs | II. Earliest life forms; absorbed organic molecules from the environment |
| C. Photoautotrophs (Anoxygenic) | III. Synthesized food using chemical energy (inorganic substrates) |
| D. Photoautotrophs (Oxygenic) | IV. Used sunlight but H2S instead of water; did not release Oxygen |
#Question id: 40220
#Applied Microbiology
Which component of a plasmid vector allows a researcher to distinguish between host cells that have taken up the plasmid and those that have not?
#Question id: 40221
#Applied Microbiology
A gene incorporated into a vector that confers a trait suitable for artificial selection of transformed cells (e.g., survival on ampicillin-containing agar) is best known as a:
#Question id: 40222
#Applied Microbiology
many cloning vectors, an antibiotic resistance gene is used for ________, while a gene like lacZ (for blue-white color change) is often used for ________.
#Question id: 40223
#Applied Microbiology
An E. coli cell that successfully takes up a pBR322 plasmid (which carries ampicillin and tetracycline resistance genes) will be able to grow on:
#Question id: 40224
#Applied Microbiology
Match the vector component in Column A with its primary function in Column B
| Column A (Component) | Column B (Function) |
| 1. ori site | a. Allows for the insertion of foreign DNA using restriction enzymes. |
| 2. Antibiotic resistance gene | b. Sequence responsible for initiating autonomous replication of the vector. |
| 3. Multiple Cloning Site (MCS) | c. Enables the identification and survival of transformed host cells under specific conditions. |