Q. Assertion: SWISS-Prot and PIR are considered secondary databases. 

Reason: They provide curated and annotated information, such as protein families and functional classifications, derived from primary data.

TLS Online TPP Program

#Question id: 38734

#Plant Biotechnology

Q.  Regarding density gradient centrifugation used in protoplast purification:

1. Separation is based on buoyant density.

2. Viable protoplasts form a distinct band.

3. Broken cells sediment faster than intact protoplasts.

4. The method separates cells based on size alone.

Which statements are correct?

TLS Online TPP Program

#Question id: 38735

#Plant Biotechnology

Q. With reference to feeder layer technique in protoplast culture:

1. Feeder cells provide diffusible growth factors.

2. It compensates for the absence of cell-to-cell signaling.

3. Feeder cells directly fuse with protoplasts.

4. It increases plating efficiency of isolated protoplasts.

Which statements are correct?

TLS Online TPP Program

#Question id: 38736

#Plant Biotechnology

Q. Regarding callus formation from protoplasts:

1. Callus formation requires prior regeneration of the cell wall.

2. Callus cells are genetically identical to the donor plant.

3. Callus represents a dedifferentiated state.

4. High auxin concentration promotes callus induction.

Which statements are correct?

TLS Online TPP Program

#Question id: 38739

#Plant Biotechnology

Q. Which of the following pairs is INCORRECTLY matched?

TLS Online TPP Program

#Question id: 38740

#Plant Biotechnology

Q. Identify the correctly matched set used commonly for plant protoplast isolation:

TLS Online TPP Program

#Question id: 38741

#Plant Biotechnology

Q. Which enzyme combination is MOST appropriate for complete degradation of primary plant cell wall during protoplast isolation?