TLS Online TPP Program

#Question id: 39891


Q. In bioinformatics, which of the following scenarios would be least suitable for using a Global Alignment algorithm (such as the Needleman-Wunsch algorithm)?

#Bio-Informatics
  1. Aligning two protein sequences that share a common ancestor and are nearly identical in length.
  2.  Identifying a highly conserved functional domain located in the middle of two otherwise unrelated, long DNA sequences.
  3. Comparing two alleles of the same gene to identify single nucleotide polymorphisms (SNPs).
  4. Mapping a 500bp sequence against another 510bp sequence where high similarity is expected throughout
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TLS Online TPP Program

#Question id: 40196

#Applied Microbiology

In a continuous culture system (Chemostat), what happens if the dilution rate (D) exceeds the maximum specific growth rate (mumax) of the organism?

TLS Online TPP Program

#Question id: 40197

#Applied Microbiology

Assertion (A): A turbidostat is most effective when operating at high dilution rates near the maximum specific growth rate (mumax}).

Reason (R): At low dilution rates, the chemostat is more stable because the concentration of the limiting nutrient is easier to control than turbidity

TLS Online TPP Program

#Question id: 40198

#Applied Microbiology

Read the following statements regarding Fed-Batch culture and choose the correct option.

Statement I: Fed-batch culture is ideal for producing secondary metabolites (like antibiotics) because it allows the culture to be maintained in the stationary phase for a prolonged period.

Statement II: In fed-batch culture, the volume of the culture broth remains constant throughout the fermentation process. 

TLS Online TPP Program

#Question id: 40199

#Applied Microbiology

Match the parameter in Column A with its formula or definition in Column B

Column A (Parameter)Column B (Definition/Formula)
1. Dilution Rate (D)A. ln (2) / mu
2. Doubling Time (td)B. Flow rate (F) / Volume (V)
3. Yield Coefficient (Y)C. Change in cell mass / Change in substrate consumed
4. Specific Growth Rate (mu)D. 1/x . dx/dt

TLS Online TPP Program

#Question id: 40200

#Applied Microbiology

Calculate the melting temperature (Tm) for the following primer sequence using the Wallace Rule

Sequence: 5'-GGCCAAAATTTTGGCC-3'

TLS Online TPP Program

#Question id: 40201

#Environmental Science

Match List I (Atmospheric Layers) with List II (Characteristics):

List I (Layer)List II (Characteristic)
A. TroposphereI. Reflects radio waves
B. StratosphereII. Lapse rate is positive (Temperature decreases with height)
C. ThermosphereIII. Contains the Ozone layer
D. IonosphereIV. Temperature increases significantly with height due to solar radiation