TLS Online TPP Program
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TLS Online TPP Program
#Question id: 38772
#Plant Biotechnology
Q. Statement I: Protoplasts are isolated by enzymatic digestion of the cell wall using a combination of cellulase and pectinase.
Statement II: Cellulose microfibrils and pectic substances are major structural components of plant cell walls.
TLS Online TPP Program
#Question id: 38773
#Plant Biotechnology
Q.Statement I: The pH of the enzyme solution for protoplast isolation is maintained between 4.7 and 8.0.
Statement II: Enzymes involved in cell wall degradation show activity only at neutral pH.
TLS Online TPP Program
#Question id: 38774
#Plant Biotechnology
Q. Statement I: Protoplast isolation is usually carried out at a temperature range of 25–30°C.
Statement II: This temperature range supports optimal enzyme activity and maintains protoplast viability.
TLS Online TPP Program
#Question id: 38775
#Plant Biotechnology
Q. Statement I: High osmotic concentration of the enzyme mixture is maintained using sorbitol or mannitol.
Statement II: Elevated osmoticum prevents protoplast bursting by balancing internal turgor pressure.
TLS Online TPP Program
#Question id: 38776
#Plant Biotechnology
Q. Statement I: Calcium chloride is added to the osmoticum during protoplast isolation.
Statement II: Calcium ions improve plasma membrane stability of protoplasts.
TLS Online TPP Program
#Question id: 38777
#Plant Biotechnology
Q. Statement I: B5 and MS media are commonly used for culturing isolated protoplasts.
Statement II: These media provide essential macro- and micronutrients required for protoplast survival and division.