TLS Online TPP Program

#Question id: 40213


In the following question, a statement of Assertion (A) is followed by a statement of Reason (R).

Assertion (A): The annealing step must be performed at a significantly lower temperature than the denaturation step.

Reason (R): High temperatures destabilize hydrogen bonds, while lower temperatures favor the formation of hydrogen bonds required for primers to hybridize with the template.

#Applied Microbiology
  1. Both A and R are true, and R is the correct explanation of A.
  2. Both A and R are true, but R is NOT the correct explanation of A.
  3. A is true, but R is false.
  4. A is false, but R is true.