In the following question, a statement of Assertion (A) is followed by a statement of Reason (R).

Assertion (A): The annealing step must be performed at a significantly lower temperature than the denaturation step.

Reason (R): High temperatures destabilize hydrogen bonds, while lower temperatures favor the formation of hydrogen bonds required for primers to hybridize with the template.

TLS Online TPP Program

#Question id: 12739

#Applied Microbiology

Method used for gene editing and for the development in Gene modifications is known as_______.

TLS Online TPP Program

#Question id: 12739

#Bio-Informatics

Method used for gene editing and for the development in Gene modifications is known as_______.

TLS Online TPP Program

#Question id: 12911

#Zoology

Match the following:

A. DNA polymerases	i. Alkaline phosphatase	a. RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase	ii. T4 DNA ligase	b. Thermostable DNA polymerase used in PCR
C. Ligases	iii. AMV-RT	c. Removes 5'-phosphates from DNA and RNA
D. Phosphatases	iv. Taq DNA pol	d. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond

TLS Online TPP Program

#Question id: 12911

#Life Sciences

Match the following:

A. DNA polymerases	i. Alkaline phosphatase	a. RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase	ii. T4 DNA ligase	b. Thermostable DNA polymerase used in PCR
C. Ligases	iii. AMV-RT	c. Removes 5'-phosphates from DNA and RNA
D. Phosphatases	iv. Taq DNA pol	d. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond

TLS Online TPP Program

#Question id: 12911

#Applied Microbiology

Match the following:

A. DNA polymerases	i. Alkaline phosphatase	a. RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase	ii. T4 DNA ligase	b. Thermostable DNA polymerase used in PCR
C. Ligases	iii. AMV-RT	c. Removes 5'-phosphates from DNA and RNA
D. Phosphatases	iv. Taq DNA pol	d. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond

TLS Online TPP Program

#Question id: 12911

#Bio-Informatics

Match the following:

A. DNA polymerases	i. Alkaline phosphatase	a. RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase	ii. T4 DNA ligase	b. Thermostable DNA polymerase used in PCR
C. Ligases	iii. AMV-RT	c. Removes 5'-phosphates from DNA and RNA
D. Phosphatases	iv. Taq DNA pol	d. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond