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TLS Online TPP Program

#Question id: 40224

Match the vector component in Column A with its primary function in Column B

Column A (Component)Column B (Function)
1. ori sitea. Allows for the insertion of foreign DNA using restriction enzymes.
2. Antibiotic resistance geneb. Sequence responsible for initiating autonomous replication of the vector.
3. Multiple Cloning Site (MCS)

c. Enables the identification and survival of transformed host cells under specific conditions.

#Applied Microbiology
  1. 1 - b , 2 - c , 3 - a
  2. 1 - c , 2 - b , 3 - a
  3. 1 - b , 2 - c , 3 - a
  4. 1 - a , 2 - c , 3 - b
More Questions
TLS Online TPP Program

#Question id: 38747

#Plant Biotechnology

Q. Assertion (A): Co-culturing is a vital technique for the regeneration of recalcitrant protoplasts. 
Reason (R): Fast-growing protoplasts in the medium secrete diffusible substances that stimulate cell wall synthesis in neighboring cells.
TLS Online TPP Program

#Question id: 38748

#Plant Biotechnology

Q. Assertion (A): In a co-culture system, the two types of protoplasts must be in direct physical contact to exchange nutrients.
 Reason (R): Growth factors provided by "nurse" cells are diffusible and can move through the liquid or semi-solid culture medium.
TLS Online TPP Program

#Question id: 38749

#Plant Biotechnology

Q. Assertion (A): Protoplasts are initially fragile and cannot undergo mitosis immediately after isolation. 
Reason (R): The enzymatic degradation of the cell wall during isolation removes the structural constraint required for organized cell division.
TLS Online TPP Program

#Question id: 38751

#Plant Biotechnology

Q.Assertion (A): Fast-growing protoplasts act as a "feeder layer" in co-culture experiments. 
Reason (R): They compete with slow-growing protoplasts for limited carbon sources, thereby forcing the slow-growing cells to adapt faster.
TLS Online TPP Program

#Question id: 38753

#Plant Biotechnology

Q. Which staining method is used specifically to detect the onset of cell wall formation in cultured protoplasts? 
TLS Online TPP Program

#Question id: 38754

#Plant Biotechnology

Q. A researcher observes protoplasts under a fluorescence microscope and sees a bright yellow-green glow. This indicates that: