TLS Online TPP Program

#Question id: 40412


Match the Reagents in List-I with their specific role/function in List-II.

List-I (Reagent)List-II (Function)
(A) Crystal Violet(I) Mordant (Fixes the dye)
(B) Gram's Iodine(II) Counterstain
(C) 95% Ethyl Alcohol(III) Primary Stain
(D) Safranin(IV) Decolorizing Agent

#Applied Microbiology
  1. (A)-(II), (B)-(IV), (C)-(I), (D)-(III)
  2. (A)-(III), (B)-(IV), (C)-(I), (D)-(II)
  3. (A)-(III), (B)-(I), (C)-(IV), (D)-(II)
  4. (A)-(I), (B)-(III), (C)-(IV), (D)-(II)
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TLS Online TPP Program

#Question id: 39282

#Plant Biotechnology

Q. Statement I: In the cybrid approach, iodoacetate is used to inactivate the nuclei of the recipient strain (Parent A). 
Statement II: Irradiation is used to inactivate the nuclei of the donor strain (Parent B) to ensure only its cytoplasm is transferred.

TLS Online TPP Program

#Question id: 39283

#Plant Biotechnology

Q. Statement I: In the fusion product described, the mitochondria are always derived from the donor strain (Parent B).
 Statement II: The chloroplasts in the resulting regenerants must always come from the recipient strain (Parent A)

TLS Online TPP Program

#Question id: 39284

#Plant Biotechnology

Q. Statement I: Fusing tomato protoplasts with irradiated Solanum acaule protoplasts produced plants that were morphologically identical to Solanum acaule. 
Statement II: The resulting tomato regenerants exhibited Cytoplasmic Male Sterility (CMS), which was inherited maternally.

TLS Online TPP Program

#Question id: 39285

#Plant Biotechnology

Q. Statement I: Protoplast fusion can be used to develop male sterile lines even between sexually incompatible species. 
Statement II: CMS lines developed through this method cannot be used with traditional CMS restorers.

TLS Online TPP Program

#Question id: 39286

#Plant Biotechnology

Q. Assertion: Cybrids can be produced by the irradiation of protoplasts from one of the fusion parents. 
Reason: Irradiation effectively inactivates the nucleus of the donor protoplast, allowing only its cytoplasm to contribute to the fusion product.

TLS Online TPP Program

#Question id: 39287

#Plant Biotechnology

Q. Assertion: Cybridization is a valuable tool for transferring traits like cytoplasmic male sterility (CMS) and herbicide resistance in crops like rice and tobacco. 
Reason: Cybrids contain the nuclear genome of only one species, which ensures the plant retains its primary species characteristics while gaining specific cytoplasmic traits.