TLS Online TPP Program

#Question id: 40442


Which of the following statements are correct regarding Crassulacean Acid Metabolism (CAM) plants?
A. They possess "Scotoactive" stomata which open during the night and close during the day.
B. The primary carboxylation enzyme during the night is RuBisCO.
C. Malic acid is stored in the vacuole during the night, leading to acidification of the cell.
D. During the day, malate is decarboxylated to release CO2 for the Calvin Cycle.
Choose the correct answer from the options given below:

#Botany
  1. A, B and C only

  2. A, C and D only

  3. B and D only
  4. A and D only

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TLS Online TPP Program

#Question id: 40422

#Applied Microbiology

Assertion (A): Sickle cell anemia is a classic example of a missense mutation.

Reason (R): In the beta-globin chain of hemoglobin, a single nucleotide substitution results in the replacement of Glutamic acid by Valine at the sixth position

TLS Online TPP Program

#Question id: 40423

#Applied Microbiology

Match the mutation type in Column I with its primary effect in Column II.

Column I (Mutation Type)Column II (Effect)
P. Nonsense1. Single amino acid change in the polypeptide.
Q. Frameshift2. Premature termination of translation.
R. Missense3. Alteration of the reading frame for all downstream codons.
S. Silent4. No change in the primary structure of the protein.

TLS Online TPP Program

#Question id: 40424

#Applied Microbiology

Which of the following components must a yeast-bacterial shuttle vector possess to be functional in both hosts?

TLS Online TPP Program

#Question id: 40425

#Applied Microbiology

Given below are two statements:

    Statement I: A shuttle vector is designed to allow the cloning of a gene in E. coli and its subsequent expression in a eukaryotic cell.

      Statement II: pBR322 is a classic example of a shuttle vector because it can replicate in both animals and bacteria.

      In the light of the above statements, choose the correct answer from the options given below:

      TLS Online TPP Program

      #Question id: 40426

      #Applied Microbiology

        Assertion (A): Shuttle vectors are naturally occurring plasmids found in Agrobacterium tumefaciens.

          Reason (R): They contain two origins of replication, enabling them to replicate in two different host organisms

          Choose the correct option: 

          TLS Online TPP Program

          #Question id: 40427

          #Applied Microbiology

          Match the Vector Type (List I) with its unique Feature/Function (List II).

          List I (Vector Type)List II (Feature/Function)
          (a) Expression Vector(i) Can accomodate large DNA inserts (100-300 kb)
          (b) Shuttle Vector(ii) Designed for protein production (transcription/translation)
          (c) BAC (Bacterial Artificial Chromosome)(iii) Replicates in two unrelated host species
          (d) Cosmid(iv) Hybrid of plasmid and phage lambda 'cos' site