Consider the following statements regarding thermal pollution:

1.It reduces dissolved oxygen levels

2.It accelerates metabolic rate of aquatic organisms

3.It always increases species diversity

4.It may cause thermal shock

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#Question id: 40204

#Applied Microbiology

Match the PCR component in Column A with its specific function in Column B

Column A (Component)	Column B (Function)
1. dNTPs	A. Provides the starting point for DNA synthesis
2. Primers	B. Building blocks for the new DNA strand
3. Buffer	C. Maintains pH and salt conditions
4. Template DNA	D. Contains the target region to be amplified

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#Question id: 40205

#Applied Microbiology

Read the following statements regarding PCR and choose the correct option.

Statement I: DNA synthesis during PCR always proceeds in the 3' ---------5' direction.

Statement II: If the annealing temperature is set too low, it often leads to non-specific binding of primers and amplification of incorrect products. 

TLS Online TPP Program

#Question id: 40206

#Applied Microbiology

Assertion (A): Taq polymerase is preferred over normal DNA polymerases (like E. coli DNA Pol I) for PCR.

Reason (R): Taq polymerase has a proofreading activity (3'-------5'exonuclease) that ensures zero errors during amplification.

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#Question id: 40207

#Environmental Science

Assertion (A): The pH of normal rain is considered to be exactly 7.0 (Neutral).

Reason (R): Carbon dioxide (CO2) in the atmosphere dissolves in raindrops to form weak carbonic acid.

TLS Online TPP Program

#Question id: 40208

#Environmental Science

Given below are two statements:

Statement I: Under the Environmental Impact Assessment (EIA) Notification 2006, "Category A" projects require clearance from the Central Government (MoEFCC).

Statement II: "Category B" projects do not require any Environmental Clearance from either State or Central authorities.

In light of the above statements, choose the correct answer:

TLS Online TPP Program

#Question id: 40209

#Applied Microbiology

The process by which ATP is synthesized using the energy released by the flow of protons (H⁺) across a membrane down their electrochemical gradient is known as: