First sugar residue is attached to dolichol by a high-energy pyrophosphate linkage & the other sugars are added by glycosidic bonds

TLS Online TPP Program

#Id: 8203

#Section 3: Genetics, Cellular and Molecular Biology

An H+ ATPase in the membrane pumps H+ into the lysosome, maintaining its lumen at an acidic pH.

TLS Online TPP Program

#Id: 8202

#Section 3: Genetics, Cellular and Molecular Biology

Most of the lysosome membrane proteins, are highly glycosylated, which helps to protect them from the lysosome proteases

TLS Online TPP Program

#Id: 8201

#Section 3: Genetics, Cellular and Molecular Biology

Lysosome an interior pH of about 4.5–5.0

TLS Online TPP Program

#Id: 8200

#Section 3: Genetics, Cellular and Molecular Biology

For optimal activity, of acid hydrolases they need to be: Activated by proteolytic cleavage Requires an acid environment.

TLS Online TPP Program

#Id: 8199

#Section 3: Genetics, Cellular and Molecular Biology

Lysosomes contain about 40 types of hydrolytic enzymes, All are acid hydrolases, work best at acidic pH including: proteases, nucleases, glycosidases, lipases, phospholipases, phosphatases, and sulfatases.

TLS Online TPP Program

#Id: 8198

#Section 3: Genetics, Cellular and Molecular Biology

Lysosomes are membrane-enclosed organelles filled with soluble hydrolytic enzymes that digest macromolecules.