M6P residues are generated in the cis-Golgi by two Golgi-resident enzymes. 

1. N-acetylglucosamine (GlcNAc) phosphotransferase
2. Phosphodiesterase

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Signal sequences

contain one or more positively charged amino acids adjacent to a continuous stretch of 6–12 hydrophobic residues (known as the hydrophobic core)

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Latrunculin, 

a toxin secreted by sponges, binds and sequesters G-actin