Fission yeast cells carrying a CDK1 mutation in which the tyrosine-15 residue is replaced by phenylalanine

show the same premature mitotic CDK activation and entry into mitosis.

TLS Online TPP Program

#Id: 8859

#Section 3: Genetics, Cellular and Molecular Biology

During G1, 

cohesins associate with chromosomes by cohesin loading factors Scc2 and Scc4 and unloaded continuously by a cohesin associated complex Pds5 and Wap1 proteins. 

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#Id: 8860

#Section 3: Genetics, Cellular and Molecular Biology

During S phase

DNA-bound G1 cohesins Convert into cohesive complexes by cohesin acetyl transferases (CoATs)

which acetylate Smc3 subunit & prevents unloading of cohesins by Pds5-Wapl, thereby stabilizing the cohesins on chromosomes.

TLS Online TPP Program

#Id: 8862

#Section 3: Genetics, Cellular and Molecular Biology

In vertebrates, stabilization of cohesins requires the cohesin-associated factor sororin.

TLS Online TPP Program

#Id: 8864

#Section 3: Genetics, Cellular and Molecular Biology

Defects of cohesin’s gene expression regulation function are the cause of a group of diseases collectively called cohesinopathies.

TLS Online TPP Program

#Id: 8865

#Section 3: Genetics, Cellular and Molecular Biology

The phosphorylation state of T14 and Y15 is controlled by a dual-specificity protein kinase known as Wee1 and a pair of dual-specificity phosphatases, Cdc25B and Cdc25C (Threonine 14 is not phosphorylated in S. pombe CDK1)

TLS Online TPP Program

#Id: 8866

#Section 3: Genetics, Cellular and Molecular Biology