15000+ GATE Biotechnology practice questions with 1-minute explanatory videos.
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TLS Online TPP Program
#Question id: 14741
#Section 5: Bioprocess Engineering and Process Biotechnology
Microbial cell recovery is carried out in a continuous disc-stack centrifuge. The centrifuge is operated at 5000 rpm for separation of baker's yeast. At a feed rate of 60 1/min, 50% of the cells are recovered. At constant speed, solid recovery is inversely proportional to flow rate. What operating speed is required to recover 90% of cells if the flow rate in the centrifuge is maintained at 601/min?
TLS Online TPP Program
#Question id: 14742
#Section 5: Bioprocess Engineering and Process Biotechnology
Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-4m3, Wr = 3x10-3m3 /kg dry sephacryl, ps =1.25 x 103 kg/m3 . The partition coefficients are kpA =0.38 and kpB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone?