III. Underground Stem modification

TLS Online TPP Program

#Id: 8629

#XL - T Zoology

APC/C works with substrate-targeting factors called Cdc20 and Cdh1

TLS Online TPP Program

#Id: 8630

#XL - T Zoology

TLS Online TPP Program

#Id: 8631

#XL - T Zoology

APC/C core is organized into three modules: 

1. a scaffold module 

2. a catalytic module

3. a substrate recognition module

TLS Online TPP Program

#Id: 8632

#XL - T Zoology

Substrate-targeting subunits of the APC/C recognize specific sequence motifs on the proteins that the APC/C ubiquitinates. One motif, the destruction box or D-box, has the consensus sequence

RXXLX[D/E]ΦΦΦXN[N/S]

TLS Online TPP Program

#Id: 8633

#XL - T Zoology

Mutating or deleting the destruction box in mitotic cyclins prevented their ubiquitination and blocked cells from exiting mitosis.

TLS Online TPP Program

#Id: 8634

#XL - T Zoology

At the metaphase to anaphase transition, APC/C bound to Cdc20 targets cyclins A /B and securin for degradation.