Nonpolar amino acids have three ionisations zones

1. 1--------pK1 – charge +1

2. pK1---------pK2 charge 0

3. pK2---------14 charge -1

TLS Online TPP Program

#Id: 7914

#XL - Q Biochemistry

Matrix-targeting sequences

Located at the N-terminus

Usually 20–50 amino acids in length

Rich in hydrophobic amino acids, positively charged basic amino acids (arginine & lysine), and hydroxylated ones (serine and threonine)

Amphipathicity of matrix-targeting sequences is critical to their function

TLS Online TPP Program

#Id: 8874

#XL - R Botany

Initial pool of active cyclin B-CDK1 phosphorylates Cdc25C, this recruits Pin1 and Plk1, which recognize Cdc25C through their phospho-binding domains. 

TLS Online TPP Program

#Id: 9308

#XL - T Zoology

RPE65 is the enzyme in the pigment epithelial cells of the retina that catalyzes the isomerization of the intermediate esterified all-trans retinol to 11-cis-retinol.

TLS Online TPP Program

#Id: 8676

#XL - R Botany

TLS Online TPP Program

#Id: 8607

#XL - R Botany

Cdk1 

=Cdc2 (vertebrates and fission yeast)

= Cdc28  (budding yeast)