When spindle microtubule elongation at the end of anaphase has correctly positioned segregating daughter chromosomes in the bud, Tem1 is converted into its active GTP-bound state, activating the protein kinase signaling cascade.

TLS Online TPP Program

#Id: 8871

#XL - T Zoology

Until Mitotic CDKs is not active, the activatory protein phosphatase, Cdc25, is phosphorylated, bound and inhibited by phosphoserine/threonine-binding protein 14-3-3.

TLS Online TPP Program

#Id: 8872

#XL - T Zoology

The entire process of mitotic CDKs activation and entry into mitosis involves two positive feedback loops and coordination between events at the centrosome, in the cytoplasm, and in the nucleus.

TLS Online TPP Program

#Id: 8873

#XL - T Zoology

Cdc25B, activating an initial small pool of cyclin B/1 at the centrosome, which is facilitate centrosome maturation.

TLS Online TPP Program

#Id: 8874

#XL - T Zoology

Initial pool of active cyclin B-CDK1 phosphorylates Cdc25C, this recruits Pin1 and Plk1, which recognize Cdc25C through their phospho-binding domains. 

TLS Online TPP Program

#Id: 8875

#XL - T Zoology

Pin1 and Plk1 modify and phosphorylate Cdc25C to stimulate its phosphatase activity

TLS Online TPP Program

#Id: 8877

#XL - T Zoology

The pool of active Cdc25C is created, that activates the large pool of cytosolic cyclin B-CDK1, which activates more cytosolic Cdc25C as part of positive feedback loop 1