Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 5241

#XL - T Zoology

Genes a, b, and c are widely spaced in the bacterial genome. Transducing phage from an a+ b+ c+ bacterium were used to infect a culture of a- b- c- cells, and b+ transductants were selected. Which of the following best describes the predicted genotypes of these transductants?

#Question id: 5242

#XL - S Microbiology

Auxotrohic bacteria plating on different medium, it is observed that colonies grow on minimal plus threonine only, no colony on minimal plus leucine and no colonies grow on minimal medium cystein only. What are the actual genotypes of auxotroph?

#Question id: 5242

#XL - T Zoology

Auxotrohic bacteria plating on different medium, it is observed that colonies grow on minimal plus threonine only, no colony on minimal plus leucine and no colonies grow on minimal medium cystein only. What are the actual genotypes of auxotroph?

#Question id: 5243

#XL - S Microbiology

Auxotroph culture (His- and Lac-) culture of E. coli was mutagenised. On what media would one spread the mutagenised cells to select prototroph for both His+  and Lac+ cells?

#Question id: 5243

#XL - T Zoology

Auxotroph culture (His- and Lac-) culture of E. coli was mutagenised. On what media would one spread the mutagenised cells to select prototroph for both His+  and Lac+ cells?

#Question id: 5244

#XL - S Microbiology

Transformation can used to map bacterial gene, which of the following statement is correct?