When electronic transition occurs from higher energy state to a lower energy state with energy difference equal to ΔE electron volts, the wavelength of line emitted is approximately equal to

TLS Online TPP Program

#Question id: 13073

#XL - S Microbiology

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;

TLS Online TPP Program

#Question id: 13075

#XL - S Microbiology

Radioisotopes must be used in RFLP and RAPD markers

TLS Online TPP Program

#Question id: 13076

#XL - Q Biochemistry

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

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#Question id: 13077

#XL - S Microbiology

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13078

#XL - S Microbiology

RFLPs have several unique advantages except this one;

TLS Online TPP Program

#Question id: 13079

#XL - S Microbiology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,