Which of the following statements about filtration sterilization are correct?

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#Question id: 12911

#XL - S Microbiology

Match the following:

A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR

C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond

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#Question id: 12912

#XL - S Microbiology

Name the enzyme which separates the double-helical DNA into single strands.

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#Question id: 12913

#XL - S Microbiology

________  involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination. 

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#Question id: 12914

#XL - S Microbiology

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________

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#Question id: 12914

#XL - Q Biochemistry

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________

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#Question id: 12915

#XL - S Microbiology

Which of the following statement is not correct about PCR.