What distinguishes a coelomate animal from a pseudocoelomate animal is that coelomates

TLS Online TPP Program

#Question id: 23181

#Unit 13. Methods in Biology

__________ type of biosensors exist to measure the concentration and location of Ca2+ and the activation state of GTPase switch proteins.

TLS Online TPP Program

#Question id: 23180

#Unit 13. Methods in Biology

FRET is very sensitive to small changes in distance and in practice is not detectable at distances greater than 

TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.

1. YFP

2. RFP

3. GFP

4. CFP

Which option below contains correct combination of flurorescent molecules that can give FRET?

TLS Online TPP Program

#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as

1.  Light emitted comes from molecules above and below the plane of focus

2. Blurred image due to superposition of fluorescent images

Which of the above statement is/ are correct?

TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?