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TLS Online TPP Program

#Question id: 10296

Which of the following statements about the oxidative pentose phosphate pathway is incorrect?

#Unit 6. System Physiology – Plant
  1. NADPH production associated with biosynthetic and stress defense reactions
  2. NADPH is a substrate for reactions that remove reactive oxygen species (ROS)
  3. The reducing power generated by the pentose phosphate pathway can be balanced by mitochondrial NADPH oxidation

  4. The first two reactions of oxidative pentose phosphate pathway—which are oxidizing reactions—are essentially reversible

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TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II
Column I                                                                          Column II
1. Texas                                                                            a. far red
2. Fluorescein                                                                   b. red
3. Rhodamine                                                                   c. blue 
4. Cy5                                                                               d. green
Which of the following option comprises all correct matches?
TLS Online TPP Program

#Question id: 23155

#Unit 13. Methods in Biology

Match the following ions in column I with their relevant dyes column II.
Column I                                                       Column II
1. K+                                                                 a. Fura-2
2. Ca2+                                                             b. SNARF-1
3. H+                                                                 c. Ion potassium green
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23152

#Unit 13. Methods in Biology

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 
1. Alkaline cells
2. Acidic cells 
3. Dead cells
4. Live cells
Which of the following options are incorrect?
TLS Online TPP Program

#Question id: 23151

#Unit 13. Methods in Biology

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?
1. Fluorescence will decrease
2.  Fluorescence will increase
3. Emission of light at higher wavelength
4. Emission of light at lower wavelength
Which of the following options are correct?
TLS Online TPP Program

#Question id: 23148

#Unit 13. Methods in Biology

There are two proteins A and B to be visualized under fluorescence microscopy. Both of them are excited with same wavelength of light but A emits longer wavelength. Hence we can conclude that 
1. A is denser molecule than B
2. B is denser molecule than A
3. A is lighter molecule than B
4. B is lighter molecule than A
Which of the following option is incorrect?
TLS Online TPP Program

#Question id: 23141

#Unit 13. Methods in Biology

Resolution of a light microscope is 0.42nm at 300 wavelength of light. If the specimen is viewed under oil what is the refractive index?