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TLS Online TPP Program

#Question id: 9150

For mountain ranges that are subjected to prevailing winds, why is the climate drier on the leeward (downwind) side?

#Unit 9. Diversity of Life Forms
  1. Deserts create dry conditions on the leeward side of mountain ranges.

  2. The sun illuminates the leeward side of mountain ranges at a more direct angle, converting to heat energy, which evaporates most of the water present.

  3. Pushed by the prevailing winds on the windward side, air is forced to rise, cool, condense, and drop its precipitation, leaving drier air to descend the leeward side.

  4. Air masses pushed by the prevailing winds are stopped by mountain ranges and the moisture is used up in the stagnant air masses on the leeward side.

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TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?
1.  It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

 Column I Column II 
 1. Texas a. far red
 2. Fluorescein b. red
 3. Rhodamine c. blue
 4. Cy5 d. green
Which of the following option comprises all correct matches?
TLS Online TPP Program

#Question id: 23155

#Unit 13. Methods in Biology

Match the following ions in column I with their relevant dyes column II.

 Column I Column II
 1. K+ a. Fura-2
 2. Ca2+ b. SNARF-1
 3. H+ c. Ion potassium green 
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23152

#Unit 13. Methods in Biology

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 
1. Alkaline cells
2. Acidic cells 
3. Dead cells
4. Live cells
Which of the following options are incorrect?