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TLS Online TPP Program

#Question id: 5301

Which of the following processes is an early step in the whole-genome shotgun approach to sequencing?

#Unit 11. Evolution and Behavior

  1. break genomic DNA at random sites

  2. map the position of cloned DNA fragments

  3. randomly select DNA primers and hybridize these to random positions of chromosomes in preparation for sequencing

  4. break genomic DNA at specific sites

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TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II
Column I                                                                          Column II
1. Texas                                                                            a. far red
2. Fluorescein                                                                   b. red
3. Rhodamine                                                                   c. blue 
4. Cy5                                                                               d. green
Which of the following option comprises all correct matches?
TLS Online TPP Program

#Question id: 23155

#Unit 13. Methods in Biology

Match the following ions in column I with their relevant dyes column II.
Column I                                                       Column II
1. K+                                                                 a. Fura-2
2. Ca2+                                                             b. SNARF-1
3. H+                                                                 c. Ion potassium green
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23152

#Unit 13. Methods in Biology

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 
1. Alkaline cells
2. Acidic cells 
3. Dead cells
4. Live cells
Which of the following options are incorrect?
TLS Online TPP Program

#Question id: 23151

#Unit 13. Methods in Biology

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?
1. Fluorescence will decrease
2.  Fluorescence will increase
3. Emission of light at higher wavelength
4. Emission of light at lower wavelength
Which of the following options are correct?
TLS Online TPP Program

#Question id: 23148

#Unit 13. Methods in Biology

There are two proteins A and B to be visualized under fluorescence microscopy. Both of them are excited with same wavelength of light but A emits longer wavelength. Hence we can conclude that 
1. A is denser molecule than B
2. B is denser molecule than A
3. A is lighter molecule than B
4. B is lighter molecule than A
Which of the following option is incorrect?