#Question id: 4103
#Unit 3. Fundamental Processes
In a biochemical experiment, you compare the products from splicing reactions carried out in vitro using three different substrates. In each case the substrate is a construct containing a single intron surrounded by two exons, and in all cases the construct is the same overall size. But in one case, the intron is a group I intron, in another a group II intron, and in the third an intron removed by the spliceosome. Each construct is labelled in a manner that allows it to be detected after gel electrophoresis, and each is tested in two reactions—one, conditions that support self-splicing, and two, in the presence of nuclear extract as well. Note that, for simplification, only the final products of the splicing reaction are seen, but before degradation of the introns.
Which of the following observation is/are correct?
A. In lane A, the presence of nuclear abstract, a band for the lariat and the spliced product and pre-mRNA.
B. Lane B shows Group I Introns and Lane C is for Group II introns and reaction is nonself-splicing
C. Lane C is Group I intron. Splicing takes place in the absence and presence of nuclear abstract, and the reaction is self-splicing.
#Question id: 3639
#Unit 3. Fundamental Processes
Replication of a bacterial chromosome normally starts at a fixed point called:
#Question id: 3640
#Unit 3. Fundamental Processes
Which of the following is true for the mechanism of excision repair?
#Question id: 3641
#Unit 3. Fundamental Processes
The point at which separation of the strands and synthesis of new DNA takes place is known as the:
#Question id: 3642
#Unit 3. Fundamental Processes
Any piece of DNA which replicates as a single unit is called
#Question id: 3643
#Unit 3. Fundamental Processes
One DNA strand, the______________, is made continuously in a 5→3 direction from the origin.