Practice Questions

TLS Online TPP Program

#Question id: 617

Compare the two reaction coordinate diagrams below and select the answer that correctly describes their relationship. In each case, the single intermediate is the ES complex.

#Unit 1. Molecules and their Interaction Relevant to Biology

(a) describes a strict “lock and key” model, whereas (b) describes a transition-state complementarity model.
The activation energy for the catalyzed reaction is #5 in (a) and is #7 in (b).
The activation energy for the uncatalyzed reaction is given by #5 + #6 in (a) and by #7 + #4 in (b).
The contribution of binding energy is given by #5 in (a) and by #7 in (b).

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TLS Online TPP Program

#Question id: 23183

#Unit 13. Methods in Biology

FRET sensors have been developed to determine where signaling between

TLS Online TPP Program

#Question id: 23181

#Unit 13. Methods in Biology

__________ type of biosensors exist to measure the concentration and location of Ca2+ and the activation state of GTPase switch proteins.

TLS Online TPP Program

#Question id: 23180

#Unit 13. Methods in Biology

FRET is very sensitive to small changes in distance and in practice is not detectable at distances greater than

TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.

Which option below contains correct combination of flurorescent molecules that can give FRET?

TLS Online TPP Program

#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as

1. Light emitted comes from molecules above and below the plane of focus

2. Blurred image due to superposition of fluorescent images

Which of the above statement is/ are correct?

TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?

Which of the following options is incorrect?