Most proteins denature at both high and low pH. At high pH, the ionization of internal tyrosines is thought to be the main destabilizing influence, whereas at low pH, the protonation of buried histidines. A titration curve for the unfolding of the enzyme ribonuclease is shown in Figure. Given the relation between the titration curves for protein unfolding and histidine protonation, which of the following conclusion can be drawn correctly?

A. The sharp transition in graph indicates a highly cooperative process; when the protein starts to unfold, it completes the process rapidly

B. The sharp transition in graph indicates a highly noncooperative process

C. Several buried histidines can ionize when the chain starts to unfold, so that when one goes, they all go together due to cooperative behaviour of protein folding

D. Buried histidine (pK of 4 in this example) becomes accessible to solvent, its pK will shift toward its normal value of 6, significantly steepening its titration curve.

#Question id: 4895

#Unit 8. Inheritance Biology

What is the difference between a linkage map and a physical map?

a) Genetic maps are based on recombination frequencies, but physical maps are based on restriction digestion.

b) Physical DNA map describes the absolute position of genes on a chromosome while genetic map so variable

c) Genetic maps are important to identify the origin of diseases, whether they are inherited or arise due to a random mutation.

#Question id: 4894

#Unit 8. Inheritance Biology

Following is figure showing restriction site on coding gene. Which of the following is mode of inheritance?

#Question id: 4893

#Unit 8. Inheritance Biology

In tomatoes, tall (D) is dominant over dwarf (d) and smooth fruit (P) is dominant over pubescent fruit (p), which is covered with fine hairs. A farmer has two tall andsmooth tomato plants, which we will call plant A and plant B. The farmer crosses plants A and B with the same dwarf and pubescent plant and obtains the following numbers of progeny

a. The genotypes of both plant A and plant B are DdPp

b. In plant A, Gene D and Gene P link as cis configuration

c. In plant B, Gene D and Gene P link as cis configuration

d. In plant A, Gene D and Gene P link as trans configuration

e. In plant B, Gene D and Gene P link as trans configuration

Which of the following above statement are not correct?

#Question id: 4892

#Unit 8. Inheritance Biology

The RFLP pattern for this locus, obtained by hybridization using a probe internal sites, from family is shown below: Based on the above profile, what is the nature of the genetic disorder?

#Question id: 4891

#Unit 8. Inheritance Biology

plantlike organism on the planet Pandora can have three recessive genetic traits: bluish leaves, due to an allele (a) of gene A; a feathered stem, due to an allele (b) of gene B; and hollow roots due to an allele (c) of gene C. The three genes are linked and recombine. A geneticist performed a testcross with an organism that had been found to be heterozygous for the three recessive traits, and she was able to identify progeny of the following phenotypic distribution (+ = wild type):

 A) Genes are in the order: a-c-b

B) Genes are in the order: a-b-c.

C) Genes are in the order: b-a-c

D) Interference is one

E) Greatest distance between gene a and b

#Question id: 4890

#Unit 8. Inheritance Biology

A strain of Neurospora crassa, unable to synthesize the vitamin thiamine (t), is crossed with a strain that cannot synthesize the amino acid arginine (a). What information can be obtained from the resultant frequencies of ascospore patterns?

 A) The two loci (a) and (t) are independent assortment of each other

B) Both genes are closely linked to their respective centromeres.

C) no crossovers occurred between either gene and its centromere.

D) These two gene may reside on different chromosomes.