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TLS Online TPP Program

#Question id: 12186

In 2001, the Nobel Prize in Physiology or Medicine was awarded to three cell cycle scientists. Paul Nurse was recognized for his studies with the fission yeast S. pombe, in particular for the discovery and characterization of the wee1+ gene. What did the characterization of the wee1+ gene tell us about cell cycle control?

#Unit 2. Cellular Organization
  1. The dual-specificity protein kinase Wee1 phosphorylates CDKs on the activatory tyrosine 15.
  2. Yeast cells with a defective wee1+ gene activate S phase CDKs prematurely and hence experience premature entry into DNA replication. 
  3. Fission yeast cells carrying a CDK1 mutation in which the tyrosine 15 residue is replaced by phenylalanine (which is structurally similar to tyrosine but cannot be phosphorylated) show the premature mitotic CDK activation and entry into mitosis.
  4. The dual-specificity protein phosphatase Wee1 dephosphorylates CDKs on the inhibitory tyrosine 15.

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TLS Online TPP Program

#Question id: 19227

#Unit 12. Applied Biology

Genetically transformed plant using the A. tumefaciens strain expressing ACC deaminase, the transformation frequency of the plants
TLS Online TPP Program

#Question id: 19226

#Unit 12. Applied Biology

A systematic examination of the conditions that are used in Agrobacterium-mediated plant transformation revealed that a hormone that significantly decreased the transfer of genes to plant genomes. It is
TLS Online TPP Program

#Question id: 19223

#Unit 12. Applied Biology

Based on the DNA sequence of a commonly used binary vector some example are
1. pBIN19
2. pCB301
3. pBR322
Which of the following above statements are correct?
TLS Online TPP Program

#Question id: 19216

#Unit 12. Applied Biology

Agrobacterium respond to which plant phenolic compounds which are excreted by susceptible wounded plants.
1. Toluene
2. Acetosyringone 
3. Hydroxyacetosyringon
Which of the option have incorrect statement?
TLS Online TPP Program

#Question id: 17085

#Unit 12. Applied Biology

Spontaneous cell fusion occurs at a very low rate. Enhanced fusion can be done by 
1. Sendai virus 
2. Polyethylene glycol
3. Ficol
4. Phenol red
Which of the above mentioned can be used for fusion experiments?
TLS Online TPP Program

#Question id: 17076

#Unit 12. Applied Biology

The various advantages of serum-free media could be
1. Improved reproducibility of results from different laboratories
2. Easier downstream processing of products from cultured cells
3. Biassays are free from interference due to serum proteins
4. They permit selective culture of diffrentiated cell types from the heterogeneous cultures
Which of the statements are true?