Following statements regarding to the molecular processes of capacitation.

A. The sperm cell membrane is altered by the removal of cholesterol by albumin proteins in the female reproductive tract.

B. Particular proteins or carbohydrates on the sperm surface are lost during capacitation.

C. The membrane potential of the sperm cell becomes more positive as potassium ions leave the sperm. This change in membrane potential may allow calcium channels to be opened and permit calcium to enter the sperm.

D. Calcium and bicarbonate ions are critical in activating cAMP production and in facilitating the membrane fusion events of the acrosome reaction.

E. As a result of cAMP formation, protein phosphorylation occurs. Once they are phosphorylated, some proteins migrate to the surface of the sperm head. One of these proteins is juno, which is critical in sperm-egg fusion.

which one of the following combinations is correct?

TLS Online TPP Program

#Question id: 23562

#Unit 13. Methods in Biology

________ is the propagation of individual host cells containing a recombinant DNA molecule.

TLS Online TPP Program

#Question id: 23561

#Unit 13. Methods in Biology

General steps in the generation of a recombinant DNA molecule in the laboratory include

TLS Online TPP Program

#Question id: 23560

#Unit 13. Methods in Biology

DNA molecules which are constructed with DNA from different sources are called

TLS Online TPP Program

#Question id: 14478

#Unit 13. Methods in Biology

Probability of throwing a number greater than 3 with an ordinary die is

TLS Online TPP Program

#Question id: 14475

#Unit 13. Methods in Biology

Probability of getting tail in a throw of a coin is

TLS Online TPP Program

#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.