TLS Online TPP Program

#Question id: 7260


There are maternally-determined variations along the animal-vegetal axis that are necessary for normal development in sea urchin. Which of the following statements about regulation in sea urchin embryos is consistent with that fact?

#Unit 5. Developmental Biology
  1. Up until the hatching of the embryo as a pluteus larva, any blastomere can be separated from the others and will regulate to go on and develop into a fully formed and full size larva.
  2. Up until the hatching of the embryo as a pluteus larva, any blastomere can be separated from the others and will regulate to go on and develop into a fully formed, yet smaller, larva.
  3. Blastomeres from the animal or vegetal poles of the embryo can be isolated and will go on to form an embryo, as long as either an animal or vegetal pole is included.
  4. Blastomeres at the 4-cell stage each still possesses a portion of the original animal-vegetal axis, and if isolated will go on to form a fully formed, yet smaller, larva.
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TLS Online TPP Program

#Question id: 5299

#Unit 8. Inheritance Biology

One particular group of mutant strains specifically required the amino acid arginine to grow, and these arginine auxotrophs formed the basis of their subsequent analysis, given in table 

TLS Online TPP Program

#Question id: 5298

#Unit 8. Inheritance Biology

Four E. coli strains of genotype a+ b− are labeled 1, 2, 3, and 4. Four strains of genotype a− b+ are labeled 5, 6, 7, and 8. The two genotypes are mixed in all possible combinations and (after incubation) are plated to determine the frequency of a+ b+ recombinants. The following results are obtained, where M = many recombinants, L = low numbers of recombinants, and 0 = no recombinants. On the basis of these results, assign a sex type (either Hfr, F+, or F−) to each strain

TLS Online TPP Program

#Question id: 5297

#Unit 8. Inheritance Biology

In a generalized transduction experiment, phages are collected from an E. coli donor strain of genotype cys+ leu+thr+ and used to transduce a recipient of genotype cys_ leu_ thr_. Initially, the treated recipient population is plated on a minimal medium supplemented with leucine and threonine. Many colonies are obtained. These colonies are then replica-plated onto three different media: What is order of gene?

TLS Online TPP Program

#Question id: 5296

#Unit 8. Inheritance Biology

A transformation experiment is performed with a donor strain that is resistant to four drugs: A, B, C, and D. The recipient is sensitive to all four drugs. The treated recipient cell population is divided up and plated on media containing various combinations of the drugs. The table below shows the results. What is the probable order of the three tightly linked genes?  

TLS Online TPP Program

#Question id: 5295

#Unit 8. Inheritance Biology

A cross is made between an Hfr that is met+ thi+ pur+ and an F-that is met- thi- pur-. Interrupted mating studies show that met+ enters the recipient last, so met+ recombinants are selected on a medium containing supplements that satisfy only the pur and thi requirements.  These recombinants are tested for the presence of the thi+ and pur+ alleles. The following numbers of individuals are found for each genotype:

A) Gene Order

I

Thi Pur  Met

II

Pur  Met  Thi

III

Pur   Thi  Met

B) Distance between Pur and Thi

IV

15.4 map unit

V

1.8 map unit

What is the following correct combination?

TLS Online TPP Program

#Question id: 5294

#Unit 8. Inheritance Biology

Interrupted mating experiments were performed using three different Hfr strains (1-3). The three strains have different combinations of selectable markers. The time of entry for markers for each strain is shown is shown in the table given.

Hfr 1

Phe

(5)

Trp

(35)

Met

(50)

Ala

(52)

Hfr 2

Trp

(2)

Val

(12)

His

(57)

Pro

(87)

Hfr 3

Ala

(10)

Met

(12)

Val

(37)

Phe

(57)

Using the above data, predict the correct sequence of markers on the E. coli chromosome