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TLS Online TPP Program

#Question id: 9581

These four Photosystem II, Cytochrome b6 f, Photosystem I and ATP synthase integral membrane complexes are vectorially oriented in the thylakoid membrane to function as follows, find out the incorrect statements;

#Unit 6. System Physiology – Plant
  1. Photosystem II oxidizes water to O2 in the thylakoid lumen and in the process releases protons into the lumen
  2. Cytochrome b6 f receives electrons from PSI and delivers them to PSII It also transports additional protons into the lumen from the stroma
  3. Photosystem I reduces NADP+ to NADPH in the stroma by the action of ferredoxin (Fd) and the flavoprotein ferredoxin–NADP reductase (FNR)
  4. ATP synthase produces ATP as protons diffuse back through it from the lumen into the stromaprotein ferredoxin–NADP reductase
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TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?
1.  It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II
Column I                                                                          Column II
1. Texas                                                                            a. far red
2. Fluorescein                                                                   b. red
3. Rhodamine                                                                   c. blue 
4. Cy5                                                                               d. green
Which of the following option comprises all correct matches?
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#Question id: 23155

#Unit 13. Methods in Biology

Match the following ions in column I with their relevant dyes column II.
Column I                                                       Column II
1. K+                                                                 a. Fura-2
2. Ca2+                                                             b. SNARF-1
3. H+                                                                 c. Ion potassium green
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23152

#Unit 13. Methods in Biology

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 
1. Alkaline cells
2. Acidic cells 
3. Dead cells
4. Live cells
Which of the following options are incorrect?
TLS Online TPP Program

#Question id: 23151

#Unit 13. Methods in Biology

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?
1. Fluorescence will decrease
2.  Fluorescence will increase
3. Emission of light at higher wavelength
4. Emission of light at lower wavelength
Which of the following options are correct?