Some given statements about Auxin signalling pathways are listed;

a) The auxin receptor is composed of two proteins: the SCF complex component TIR1 and the repressor protein AUX/IAA

b) The binding of auxin to its receptor complex initiates ubiquitin-dependent degradation of the AUX/ IAA repressor protein by the 26S proteasome 

c) TIR1 recruits AUX/ IAA proteins to the SCFTIR1  complex in an auxin-independent manner

d) AUX/IAA proteins are ubiquitinated by the E3 ligase activity of the SCFTIR1 complex, which marks the protein for destruction by the 26S proteasome

Which of the following is correct explanation of Auxin signalling pathways?

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#Question id: 19844

#Unit 13. Methods in Biology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

COLUMN I	COLUMN II
A) SYBR green	i) Annealing
B) Taqman Assay	ii) Denaturation
C) Molecular beacon	iii) pH
	iv) Amplification
	v) Extension

TLS Online TPP Program

#Question id: 19845

#Unit 13. Methods in Biology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

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#Question id: 19846

#Unit 13. Methods in Biology

Variants of PCR has been developed on the basis of___

TLS Online TPP Program

#Question id: 19865

#Unit 13. Methods in Biology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

COLUMN I	COLUMN II
A) Enhance efficiency	i) Multiplex PCR
B) Sequence specific amplification	ii) Hot-start PCR
C) Mutational analysis	iii) Megaprimer PCR
	iv) qPCR with SYBR
	v) AS-PCR
	vi) RLM-RACE

TLS Online TPP Program

#Question id: 19866

#Unit 13. Methods in Biology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?

TLS Online TPP Program

#Question id: 19867

#Unit 13. Methods in Biology

Which of the following variants of PCR were devised to prevent non-specific amplification at low temperature?