You have isolated a set of five yeast mutants that form dark red colonies instead of the usual white colonies of wild-type yeast. You cross each of the mutants to a wild-type haploid strain and obtain the results shown below;

you cross each haploid mutant strain to a different haploid mutant of the opposite mating type. From the results shown below deduce as much as you can about which mutations lie in the same gene. Clearly state any remaining ambiguities and suggest some general ways that the ambiguities might be resolved

a) That mutants 1 and 3 form one complementation group and are mutations in the same gene (gene A) 

b) That mutations 2 and 5 form a second complementation group and are mutations in a second gene (gene B).

c) That mutations 3 and 5 form a second complementation group and are mutations in a second gene (gene B).

d) The first ambiguity is whether mutant 4 has a mutation in gene A or B, or whether it represents a unique gene.

Which of the following is the correct prediction about mutants?

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#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as

1.  Light emitted comes from molecules above and below the plane of focus

2. Blurred image due to superposition of fluorescent images

Which of the above statement is/ are correct?

TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?

TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

Column I                                                                          Column II

1. Texas                                                                            a. far red

2. Fluorescein                                                                   b. red

3. Rhodamine                                                                   c. blue 

4. Cy5                                                                               d. green

Which of the following option comprises all correct matches?

TLS Online TPP Program

#Question id: 23155

#Unit 13. Methods in Biology

Match the following ions in column I with their relevant dyes column II.

Column I                                                       Column II

1. K+                                                                 a. Fura-2

2. Ca2+                                                             b. SNARF-1

3. H+                                                                 c. Ion potassium green

Which of the following options is correct?